Date of Award

11-2012

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Seema Bharathan, Ph.D.

Second Advisor

Narayanaswamy Bharathan, Ph.D.

Third Advisor

Cuong Diep, Ph.D.

Abstract

Rhizoctonia solani (teleomorph Thanatephorus cucumeris) is a worldwide distributed soil-borne ubiquitous plant pathogenic fungus that is genetically diverse. It is a major cause of death for most seedlings in cultivated crop plants. The genetic elements of viral dsRNA associated with basidiomycete R. solani, the etiology, epidemiology, and molecular biology of the disease have been conducted extensively. In this study, we extracted dsRNA segments from the AG 13 culture. The full-length of dsRNA has been synthesized using the conventional RT-PCR FLAC method, and was cloned into vector and sequenced. A new design of real time one step RT-PCR TaqMan probe for detection of dsRNA has been created. This assay was optimized specifically for RB 357 dsRNA, by testing different AGs R. solani with or without dsRNA, and two samples of binuclear Rhizoctonia. However, misdetection occurred on RB 303, it is suspected that this was because of two equal size dsRNA.

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