Date of Award

5-2015

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

N. Bharathan, Ph.D.

Second Advisor

Carl S. Luciano, Ph.D.

Third Advisor

John C. Ford, Ph.D.

Fourth Advisor

Seema Bharathan, Ph.D.

Abstract

The pathogenicity of microorganisms has been attributed to specific biomolecules produced by these microorganisms, Siderophores are low molecular weight, high affinity ferric iron chelators, which are synthesized and secreted by many microorganisms in response to iron deprivation. These compounds solubilize and bound iron and transport it back into the microbial cells. My goal was to find out if Siderophores are responsible for the pathogenicity of Rhizoctonia solani, which is a soil borne plant pathogen. To study this, five isolates of Rhizoctonia solani were selected depending on their differences in virulence, each isolate was cultured and the resulting metabolic footprints were examined using chromatographic, spectrophotometric, and chemical methods. The amount of Siderophore produced by each selected isolate was detected and quantified. It was possible to draw correlation that, the amount of Siderophore produced is directly proportional to the pathogenicity of the isolates.

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