Date of Award

Spring 5-2016

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

N. Bharathan, Ph.D.

Second Advisor

Seema Bharathan, Ph.D.

Third Advisor

Cuong Diep, Ph.D.

Fourth Advisor

Joseph Horzempa, Ph.D.

Abstract

Amongst genetically isolated anastomosis groups (AG’s), Rhizoctonia solani, a soil-borne, plant pathogenic fungus, shows increased genetic variability between these groups. Sub-groups of these AG’s can be further differentiated via colony morphology, virulence, genetic sequences, biochemical characteristics and host ranges. Data from specific protocols and standard operating procedures developed for protein purifications of filamentous fungi will be presented. Proteins were purified from select R. solani isolates that included wild-type, heterokaryons, and compared to those of reduced genomic complement, the homokaryons. All high-quality proteins were subjected to Agilent Protein Chip Technology protein separation and 2D-protein profiling. Preliminary results suggest considerable variation in the protein from the homokaryon and the wild-type isolates. ProteinsSpot statistics from 2D-gel preparations indicated nearly 81% of the proteins were similar while an under expression of 10.4% in the wild-type and 8.7% over-expression in the homokaryon exists. Additional comparisons are being made between viral-infected versus non-infected R. solani isolates.

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