Date of Award

Summer 8-2016

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Narayanaswamy Bharathan, Ph.D.

Second Advisor

Seema Bharathan, Ph.D.

Third Advisor

Daniel Widzowski, Ph.D.

Abstract

Rhizoctonia solani anastomosis group 3 (AG-3) are pathogenic to plants in the family Solanaceae. The fungus has a destructive life lifestyle therefore poses as a serious threat to the potato industry. The identification of its proteins and associated function will provide insight in devising an effective control strategy thereby ensuring food security. The purpose of this study is to identify proteins signature from virulent and a-virulent strains of Rhizoctonia solani. In this study, two isolates of Rhizoctonia solani was used. Each isolate was inoculated in soil and potato seeds were grown in them. The two isolates were also subjected to a tissue culture experiment with potato seeds. Total proteins was extracted after each treatment and their concentrations were determined. Protein extracts were also differentiated by size using protein chip analysis. Three protein extracts were selected and subjected to 2 Dimensional gel Analysis and Mass spectrophotometry for protein identification.

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