Date of Award
12-2012
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
Seema Bharathan, Ph.D.
Second Advisor
Narayanaswamy Bharathan, Ph.D.
Third Advisor
Cuong Diep, Ph.D.
Abstract
Rhizoctonia solani is a soil-borne plant pathogenic fungus with a wide host-range. The fungus primarily infects the seeds, hypocotyls, and roots, but is also capable of infecting other plant parts such as fruits, leaves and stems. Viruses infecting these fungal isolates contain double-stranded (ds) RNA genomes that have been implicated in transmissible cytoplasmic hypovirulence (TCH), which could be used as a biopesticide to control infection due to the fungus. The proposed project will establish sequence-independent in vitro amplification of Middle (M)-size viral dsRNA's implicated in TCH and subsequent characterization involving sequencing, and nucleic acid hybridization regulation of gene expression. It is hypothesized that the specific segments of dsRNA in some isolates might be involved in reduced infection. The goals of this research are to develop an optimized method to synthesize cDNA and characterize the proteins associated with the virus infecting the fungi and R. solani isolates and the fungus.
Recommended Citation
Siew, Chin Hong, "Strategic Approaches to cDNA Synthesis and Comparative Proteomics of Double-Stranded (ds) RNA Infecting Selected Rhizoctonia Solani Isolates" (2012). Theses and Dissertations (All). 1090.
https://knowledge.library.iup.edu/etd/1090