Author

Ahana Byne

Date of Award

Summer 8-2018

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Narayanaswamy Bharathan, Ph.D.

Second Advisor

Seema Bharathan, Ph.D.

Third Advisor

Robert Major, Ph.D.

Abstract

Rhizoctonia solani is a soil-borne plant pathogenic fungus causing important diseases in cultivated crop plants. R. solani is a complex species recognized by its ability to anastomose. Till today 14 anastomosis groups (AG’s) have been recognized. AG subgroups are further differentiated via genetic sequences, colony morphology, biochemical characteristics, virulence and host ranges. Many isolates of R. solani exhibit varying degree of pathogenicity on the host they infect. My researches focus on two isolates Rhs 1AP and Rhs 123E that are virulent and a-virulent, respectively. Both the isolates belong to AG-3 with Rhs 1AP containing 3.4 kb double stranded (ds) RNA that is lacking in Rhs 123E. The quantitative alteration of protein was investigated using a combination of protein identification tools such as Polyacrylamide gel electrophoresis (SDS-PAGE), protein chip, 2D-Gel and mass spectrophotometry that helped to compare protein from the selected isolates.

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