Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Daniel Widzowski

Second Advisor

Seema Bharathan

Third Advisor

Robert Major


High histamine concentrations in foods may induce a food intolerance and weight gain, but it is not clear if this is from local effects of histamine in the gastrointestinal tract or systemic effects of histamine absorbed and distributed around the body. These studies tested the systemic hypothesis by administering intraperitoneal injections of histamine or its precursor histidine for four weeks to female or male mice fed a low (10%) or high (45%) fat diet. Sub-chronic histamine or histidine resulted in no weight gain in female mice but caused weight loss in male mice fed the 45% fat diet. Histidine-injected mice had significantly reduced perigonadal fat pad mass versus controls, as did histamine-injected male mice. Serum triglycerides were increased in vehicle-injected mice fed the 45% fat diet, but were reduced in histamine or histidine-injected mice fed the 45% fat diet. These results do not support the systemic histamine hypothesis.